Anthocyanins accumulation and molecular analysis of correlated genes by metabolome and transcriptome in green and purple asparaguses (Asparagus officinalis, L.). (15th January 2019)

• Record Type: Journal Article
• Authors: Dong, Tingting; Han, Rongpeng; Yu, Jiawen; Zhu, Mingku; Zhang, Yi; Gong, Ying; Li, Zongyun
• Is Part Of: Food chemistry. Volume 271(2019)
• Page Start: 18
• Page End: 28
• Publication Date: 2019-01-15
• Subjects: 4CL 4-coumarateCoA ligase -- ANS anthocyanidin synthase -- C4H cinnamate 4-hydroxylase -- CHI chalcone isomerase -- CHS chalcone synthase -- DFR dihydroflavonol reductase -- DHK dihydrokaempferol -- F3H flavone 3-hydroxylase -- F3′H flavonoid 3′-hydroxylase -- F3′5′H flavonoid 3′, 5′-hydroxylase -- HPLC high-performance liquid chromatography -- MRM multiple reaction monitoring -- PAL phenylalanine ammonia-lyase -- UFGT flavonoid-3-O-glucosyltransferase -- qRT-PCR quantitative real-time PCR; Asparagus -- Anthocyanin -- Transcriptome -- Flavonoid biosynthesis -- Differentially expressed genes; Cyanidin 3-O-rutinoside (PubChem CID: 441674) -- Cyanidin 3-O-glucoside (PubChem CID: 44256715) -- Cyanidin 3, 5-O-diglucoside (PubChem CID: 441688) -- Pelargonidin 3-O-beta-d-glucoside (PubChem CID: 443648) -- Peonidin (PubChem CID: 441773) -- Delphinidin (PubChem CID: 128853) -- Malvidin 3-O-glucoside (PubChem CID: 443652) -- Cyanidin (PubChem CID: 128861) -- Pelargonidin (PubChem CID: 440832) -- Malvidin (PubChem CID: 159287); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.07.120
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: At least 15 kinds of anthocyanins were identified three purple asparaguses. Peonidin and cyanidin were the major anthocyanins in purple asparagus. Anthocyanins accumulation in asparagus were light-dependent. Thousands of different expressed genes were found in green and purple asparagus. One APL-like gene were inhibited greatly in both green and light-treated asparagus. Abstract: Asparagus ( A. officinalis L.) is a highly nutrition vegetable crop. Here, three purple asparagus cultivars, namely, Jing Zi-2, Purple Passion and Pacific Purple, and one green cultivar, namely, Jing Lv-1 were studied. At least 16 kinds of anthocyanins were identified in purple and green cultivars, and peonidin, cyanidin and their glycoside derivatives were found to be the major anthocyanins. Transcriptome data showed that most anthocyanin biosynthetic genes and at least 5 kinds of transcription factors were significantly differentially expressed significantly between the green and purple cultivars. Dark-treated experiments revealed that anthocyanins are not produced in the absence of light, and both the anthocyanin biosynthetic and regulatory genes were down-regulated greatly in the dark, implying that anthocyanins accumulation in asparagus is light-dependent. Overall, the results of this study provide useful information for understanding anthocyanin accumulation and the molecular mechanism of anthocyanin biosynthesis in asparagus.