Rapidly detecting major peanut allergen-Ara h2 in edible oils using a new immunomagnetic nanoparticle-based lateral flow assay. (15th January 2019)

• Record Type: Journal Article
• Authors: Yin, Hsin-Yi; Fang, Tony J.; Li, Yi-Ting; Fung, Yang-Fan; Tsai, Wen-Che; Dai, Hong-Yu; Wen, Hsiao-Wei
• Is Part Of: Food chemistry. Volume 271(2019)
• Page Start: 505
• Page End: 515
• Publication Date: 2019-01-15
• Subjects: Peanut allergen -- Ara h2 -- Immunomagnetic nanoparticle -- Lateral flow assay; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.07.064
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An IMNP-based LFA was developed for specifically detecting Ara h2 in edible oils. The assay had good specificity in identifying Ara h2 from various seeds and nuts. The visual detection limit of this assay is 0.1 mg/kg of Ara h2 in oil. Including a sample preparation step, the entire assay can be done within 5 h. Among 7 commercial peanut oils, two were identified to have Ara h2. Abstract: Ara h2 is a major peanut allergen that induces rashes, vomiting, diarrhea, and anaphylactic shock. Since peanut is a major source in producing edible oils globally, Ara h2 residues can be present in various edible oils. In this work, an immunomagnetic nanoparticle-based lateral flow assay for identifying Ara h2 in edible oils is developed. This assay exhibits high sensitivity with a visual detection limit of 0.1 mg/kg Ara h2 in oil, and favorable specificity in differentiating peanut from seeds and nuts. The calculated CV values of intra- and inter-assay were 6.73–10.21% and 4.75–8.57%, respectively, indicating high reproducibility. In an analysis of 26 oil products, Ara h2 was detected in two peanut oils as 0.122 ± 0.026 mg/kg and 0.247 ± 0.027 mg/kg. The entire method takes 5 h, including a 3.5-h sample preparation. Hence, this method has the potential to be an effective way to screen edible oils for Ara h2.