A shotgun method for high throughput screening microcystins in Margarya melanioides on a triple quadrupole tandem mass spectrometry. (15th December 2018)

• Record Type: Journal Article
• Authors: Li, Shiyan; Cui, Yiwei; Wang, Yang; Dai, Zhiyuan; Shen, Qing
• Is Part Of: Food chemistry. Volume 269(2018)
• Page Start: 89
• Page End: 95
• Publication Date: 2018-12-15
• Subjects: Shotgun -- Microcystins -- Mass spectrometry -- Precursor ion scan -- M. melanioides; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.07.004
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A shotgun method was developed for rapid screening of microcystins. The unique fragment of m / z 135.2 was used for PreIS seven microcystins. The microcystins were doubly charged when 0.1% formic acid was added in mobile phase. The recoveries ranged from 69.3 to 87.9%, and the LOD and LOQ were below 3.1 and 9.3 ng·mL −1 . 38 out of 60 samples were found to be contaminated with microcystins. Abstract: A selective and sensitive procedure for determining seven hazardous microcystins in Margarya melanioides, using tandem mass spectrometry, is presented. The product ion m / z 135.2 was selected as representative for precursor ion scanning and used to increase overall sensitivity of this shotgun method. The method was validated, and recoveries ranged from 69.3 to 87.9%, with the relative standard deviation less than 10%. Limits of detection and quantitation were less than 3.1 and 9.3 ng·mL −1, respectively. A total of 60 samples were tested using this method. 38 samples were found to be contaminated with microcystins, among which there are 31 samples whose estimated daily intake (EDI) was 0–7.8 times higher than the tolerable daily intake (TDI). This method would be useful for carrying out direct, ultra-rapid quantification of microcystins in foods, and is of significant interest for environmental and food safety applications.