Optimization of an extraction method for the simultaneous quantification of sixteen polyphenols in thirty-one pulse samples by using HPLC-MS/MS dynamic-MRM triple quadrupole. (15th November 2018)

• Record Type: Journal Article
• Authors: Caprioli, Giovanni; Nzekoue, Franks Kamgang; Giusti, Federica; Vittori, Sauro; Sagratini, Gianni
• Is Part Of: Food chemistry. Volume 266(2018)
• Page Start: 490
• Page End: 497
• Publication Date: 2018-11-15
• Subjects: DAD diode array detection -- ESI electrospray ionization -- EtOH ethanol -- FIA flow injection analysis -- GC–MS gas chromatography-mass spectrometry -- HCl Hydrochloric acid -- HPLC high performance liquid chromatography -- LOD limit of detection -- LOQ limit of quantification -- MS mass spectrometry -- MS/MS tandem mass spectrometry -- MRM multiple reaction monitoring -- NaOH sodium hydroxide -- PTFE polytetrafluoroethylene -- RP reverse phase -- RSD relative standard deviations -- SSE signal suppression enhancement -- PUFAs polyunsaturated fatty acids; Cyanidin 3-glucoside chloride (PubChem: CID 197081) -- Delphinidin 3, 5-diglucoside chloride (PubChem: CID 10100906) -- Kaempferol 3-glucoside (PubChem: CID 25203515) -- Gallic acid (PubChem: CID 370) -- Catechin (PubChem: CID 73160) -- (−)-Epicatechin (PubChem: CID 72276) -- Caffeic acid (PubChem: CID 689043) -- Chlorogenic acid (PubChem: CID 1794427) -- Syringic acid (PubChem: CID 10742) -- Rutin (PubChem: CID 5280805) -- p-Coumaric acid (PubChem: CID 637542) -- Ferulic acid (PubChem: CID 445858) -- Shikimic acid (PubChem: CID 8742) -- 3, 5-di-O-Caffeoylquinic acid (PubChem: CID 6474310) -- Vanillic acid (PubChem: CID 8468) -- Quercetin (PubChem: CID 5280343); Pulses -- Polyphenols -- Extraction methods -- HPLC-MS/MS -- Triple quadrupole; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.06.049
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: HPLC-MS/MS method for the analysis of polyphenols in pulses. Acidic hydrolysis at pH 2 was the best extraction method for pulses polyphenols. The highest levels of total polyphenols were found in beans. Black lentils and quality gold lentils displayed high level of polyphenols. Abstract: Pulses are an important source of proteins, carbohydrates, and dietary fibre, and also contain polyphenols, which are bioactive compounds with antioxidant activity. A new analytical method that uses high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) was developed for the quantification of sixteen polyphenols in thirty-one pulse varieties. Different extraction procedures were tested (acidic hydrolysis, alkaline hydrolysis and extraction without hydrolysis), and acidic hydrolysis at pH 2, extraction temperature of 20 °C and extraction time of 2 h was proven to be the best in terms of recovery percentages (99.7–107.6%). The highest polyphenol levels were found in beans, particularly black beans (459 mg kg −1 ) and ruviotto beans (189 mg kg −1 ); significant levels of polyphenols were also observed in lentils, particularly black lentils (137 mg kg −1 ) and quality gold lentils (132 mg kg −1 ). This study provides new information about legume polyphenols, offering reasons to promote legumes as part of a healthy diet.