Provitamin A–biofortified maize increases serum β-carotene, but not retinol, in marginally nourished children: a cluster-randomized trial in rural Zambia,. Issue 1 (11th May 2016)

• Record Type: Journal Article
• Authors: Palmer, Amanda C; Siamusantu, Ward; Chileshe, Justin; Schulze, Kerry J; Barffour, Maxwell; Craft, Neal E; Molobeka, Ngosa; Kalungwana, Ng'andwe; Arguello, Margia A; Mitra, Maithilee; Caswell, Bess; Klemm, Rolf DW; West, Keith P
• Is Part Of: American journal of clinical nutrition. Volume 104:Issue 1(2016)
• Page Start: 181
• Page End: 190
• Publication Date: 2016-05-11
• Subjects: beta-carotene -- biofortification -- deficiency -- vitamin A -- serum retinol -- provitamin A -- Zambia; Diet therapy -- Periodicals; Nutrition -- Periodicals; Dietetics -- Periodicals; 613.205
• DOI: 10.3945/ajcn.116.132571
• Languages: English
• ISSNs: 0002-9165

Abstract

ABSTRACT: Background: Vitamin A deficiency remains a nutritional concern in sub-Saharan Africa. Conventionally bred maize hybrids with high provitamin A carotenoid concentrations may have the potential to improve vitamin A status in maize-consuming populations. Objective: We evaluated the efficacy of regular provitamin A carotenoid–biofortified "orange" maizemeal (∼15 μg β-carotene/g) consumption in improving vitamin A status and reducing vitamin A deficiency in children. Design: This was a cluster-randomized controlled trial in the rural farming district of Mkushi, Zambia. All 4- to 8-y-old children in an ∼400-km 2 area were identified and grouped by proximity into clusters of ∼15–25 children. We randomly assigned clusters to 1 ) orange maizemeal ( n = 25), 2 ) white maizemeal ( n = 25), or 3 ) a parallel, nonintervention group ( n = 14). Children in intervention clusters ( n = 1024) received 200 g maizemeal for 6 d/wk over 6 mo; the maizemeal was prepared according to standardized recipes and served in cluster-level kitchens. Staff recorded attendance and leftovers. We collected venous blood before and after the intervention to measure serum retinol, β-carotene, C-reactive protein, and α1 -acid glycoprotein. Results: Intervention groups were comparable at baseline, and vitamin A status was better than anticipated (12.1% deficient on the basis of serum retinol <0.7 μmol/L). Although attendance at meals did not differ (85%), median daily maize intake was higher in white (154 g/d) than in orange (142 g/d) maizemeal clusters. At follow-up, mean serum β-carotene was 0.14 μmol/L (95% CI: 0.09, 0.20 μmol/L) higher in orange maizemeal clusters ( P < 0.001), but mean serum retinol (1.00 ± 0.33 μmol/L overall) and deficiency prevalence (17.1% overall) did not differ between arms. Conclusion: In this marginally nourished population, regular biofortified maizemeal consumption increased serum β-carotene concentrations but did not improve serum retinol. This trial was registered at clinicaltrials.gov as NCT01695148.