Anti-inflammatory action of two novel peptides derived from peanut worms (Sipunculus nudus) in lipopolysaccharide-induced RAW264.7 macrophages. Issue 1 (18th December 2019)

• Record Type: Journal Article
• Authors: Sangtanoo, Papassara; Srimongkol, Piroonporn; Saisavoey, Tanatorn; Reamtong, Onrapak; Karnchanatat, Aphichart
• Is Part Of: Food & function. Volume 11:Issue 1(2020)
• Page Start: 552
• Page End: 560
• Publication Date: 2019-12-18
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c9fo02178g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Peanut worm ( Sipunculus nudus Linn.) protein was hydrolyzed by three proteases and NO scavenging activity of the protein hydrolysates were evaluated. Abstract : Peanut worm ( Sipunculus nudus Linn.) protein was hydrolyzed by three proteases, and NO scavenging activity of the protein hydrolysates was evaluated. The hydrolysate obtained using Alcalase® showed the highest NO scavenging activity. This hydrolysate was fractionated using 10-, 5-, and 3 kDa molecular weight cut-off membranes, and the lowest MW fraction (<3 kDa) exhibited the highest NO scavenging activity. The <3 kDa fraction was further purified by gel filtration and high-performance liquid chromatographies. The peptides in the HPLC fraction with the strongest anti-NO activity were identified by quadrupole-time-of-flight mass spectrometry as LSPLLAAH (821.48 Da) and TVNLAYY (843.42 Da). Both peptides and the corresponding pure synthetic peptides inhibited NO production by RAW 264.7 macrophages without cytotoxicity. Quantitative real-time RT-PCR analysis showed that peptides LSPLLAAH and TVNLAYY reduced expression of proinflammatory cytokine genes iNOS, IL-6, TNF-α, and COX-2 in RAW 264.7 macrophages, suggesting that these peptides are novel anti-inflammatory candidates.