The influence of emulsion parameters on physical stability and rheological properties of Pickering emulsions stabilized by hordein nanoparticles. (April 2020)

• Record Type: Journal Article
• Authors: Boostani, Sareh; Hosseini, Seyed Mohammad Hashem; Golmakani, Mohammad-Taghi; Marefati, Ali; Abdul Hadi, Nabilah Binti; Rayner, Marilyn
• Is Part Of: Food hydrocolloids. Volume 101(2020)
• Publication Date: 2020-04
• Subjects: Hordein -- Pickering emulsion -- HIP -- Viscoelastic properties -- Emulsion parameters; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2019.105520
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: In this study, the effect of pH (3–8), ionic strength ( I, 0.01–1 M), protein particle concentration ( C, 0.5%–2%), and dispersed phase volume fraction ( Φ, 0.25–0.8) on the efficacy of hordein-based colloidal nanoparticles in the stabilization of Pickering structures was investigated. Colloidal particles were synthesized via anti-solvent method. Depending on pH and I, the average size and zeta-potential of hordein particles ranged from 425 to around 4000 nm, and +27.5 to −15.63 mV, respectively. The intense aggregation of particles at pH close to isoelectric point and also at high I led to poor formation and stability of Pickering emulsions. The overrun value was influenced by various emulsion parameters. At pH 3, triphasic (with the overrun value of 75%) and high internal phase (HIP) emulsions with an average droplet size of 15.6–86.7 μm were formed at Φ 0.25 and 0.8, respectively. At Φ 0.5 and 0.8, an elastic gel-like behavior (i.e., storage modulus ( G ′) > loss modulus ( G ″)) was observed over the applied frequency range. At Φ 0.25, a predominant viscous character ( G "> G ′) was observed at low frequencies; while, at higher frequencies, G ′ exceeded G ″ due to the foam collapse. The morphology was studied by optical microscopy and confocal laser scanning microscopy (CLSM). Monitoring the physical stability using static multiple light scattering (MLS) revealed three destabilization profiles of foam-like, emulsion-foam and gel-like structures. The results of this study might help in designing triphasic Pickering systems (using plant-based proteins) and developing solid-like structured vegetable oils from the gel-like HIP emulsions. Graphical abstract: Image 1 Highlights: pH and ionic strength affected size and zeta-potential of hordein-based particles. Particle concentration and oil fraction affected physical stability of emulsions. Hordein particles developed stable high internal phase (HIP) Pickering emulsions. Triphasic to gel-like (HIP) systems were developed at different conditions.