Influence of low soil nitrogen and phosphorus on gluten polymeric and monomeric protein distribution in two high quality spring wheat cultivars. (January 2020)

• Record Type: Journal Article
• Authors: Tóth, Brigitta; van Biljon, Angeline; Labuschagne, Maryke
• Is Part Of: Journal of cereal science. Volume 91(2020)
• Publication Date: 2020-01
• Subjects: Glutenin -- Gliadin -- Protein content -- Fertilizer; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2019.102867
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: The effect of low levels of nitrogen, phosphorus and a combination of the two on the distribution of polymeric and monomeric proteins in two high quality spring bread wheat cultivars was investigated for two consecutive seasons. Size exclusion-high performance liquid chromatography (SE-HPLC) was used to determine the quantity and relationships of monomeric and polymeric proteins, and their relationship with flour protein content (FPC) and SDS sedimentation volume (SDSS). The low nitrogen and combined low nitrogen and low phosphorus treatments had a much larger effect on the protein fractions than the low phosphorus treatment alone. The SDS-soluble large monomeric protein fraction and the percentage SDS-insoluble monomeric proteins, were significantly increased under low nitrogen and a combination of low nitrogen and low phosphorus treatments. The percentage SDS-insoluble large and total polymeric proteins was significantly reduced under low nitrogen and a combination of low nitrogen and phosphorus treatments. The SDS-soluble and -insoluble small polymeric proteins were significantly increased under both low nitrogen and a combination of low nitrogen and low phosphorus treatments. The low nitrogen treatment consistently caused the lowest FPC and SDSS values. Under low nitrogen conditions, there was a significant positive correlation between the SDS-soluble gliadins and SDSS, and FPC. Graphical abstract: Image 10889 Highlights: Low N and P levels were applied to two excellent quality wheat cultivars. SE-HPLC indicated that the low N treatment largely affected protein composition. Monomeric proteins were increased due to low N and combined low N and P treatments. Polymeric proteins were reduced due to low N and combined low N and P treatments. Low N and combined low N and P treatments significantly reduced FPC and SDSS.