Consecutive high-pressure and enzyme assisted fractionation of blackberry (Rubus fruticosus L.) pomace into functional ingredients: Process optimization and product characterization. (15th May 2020)

• Record Type: Journal Article
• Authors: Kitrytė, Vaida; Narkevičiūtė, Aistė; Tamkutė, Laura; Syrpas, Michail; Pukalskienė, Milda; Venskutonis, Petras Rimantas
• Is Part Of: Food chemistry. Volume 312(2020)
• Publication Date: 2020-05-15
• Subjects: Blackberry pomace -- Supercritical carbon dioxide extraction -- Pressurized liquid extraction -- Enzyme-assisted extraction -- Antioxidant capacity -- Phytochemicals; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.126072
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Blackberry pomace biorefining process was optimized by multistep extractions. Supercritical carbon dioxide extraction yielded 9.9% of lipophilic substances. Pressurized liquid extraction effectively recovered higher polarity compounds. Lipophilic pomace extracts contained polyunsaturated fatty acids. Polar pomace extracts contained valuable phenolic antioxidants. Abstract: In this study supercritical carbon dioxide (SFE-CO2 ) and pressurized liquid (PLE) extractions were optimized for the recovery of valuable fractions from blackberry pomace. Consecutively applied SFE-CO2 and PLE at optimized parameters yielded 9.9, 26.3 and 5.1 g/100 g of CO2, ethanol (EtOH) and water-soluble extracts, respectively. Oil of lipophilic fraction was composed mainly of healthy polyunsaturated fatty acids (linoleic 64.1%, α-linolenic 12.9%), while polar solvents effectively recovered antioxidants (up to 29.1 mg gallic acid and 168.7 mg Trolox equivalents from g pomace). PLE-EtOH extract contained 12.2 mg/g of cyanidin-3-glucoside, while other anthocyanins were detected in significantly lower quantities (0.5–0.7 mg/g). SFE-CO2 and PLE reduced the antioxidant capacity of starting plant material by 86–93%. In terms of extraction time, solvent consumption, total yields, and phytochemical characteristics, high-pressure fractionation was more efficient for obtaining valuable pomace constituents as compared to conventional and enzyme-assisted extractions.