Cellular biological activity and regulation of gene expression of antioxidant dietary fibre fraction isolated from blackcurrant incorporated in the wholemeal cereals cookies. (15th May 2020)

• Record Type: Journal Article
• Authors: Mofasser Hossain, A.K.M.; Brennan, Margaret A.; Guo, Xinbo; Zeng, Xi-An; Brennan, Charles S
• Is Part Of: Food chemistry. Volume 312(2020)
• Publication Date: 2020-05-15
• Subjects: Antioxidant-DF -- Phenolic acid -- Cellular antioxidant analysis -- Antiproliferation -- Inflammatory cytokine -- Satiety gene; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125829
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Blackcurrant incorporated fibre fraction exerted higher free and bound phenolics. Ferulic, catechin and quercetin were identified major phenolic acids. Cellular and chemical antioxidant capacity illustrated the benefits of antioxidant-DF. Blackcurrant incorporated treatment yielded control on cell proliferation. Improved regulation of satiety and inflammatory cytokine gene expression. Abstract: Free and bound phenolics were extracted from the fibre fraction of wholemeal (W) wheat and barley (B) cookies which had been fortified with 15% blackcurrant powder. Blackcurrant enriched cookies contained between 55 and 66 % higher total phenolics respectively compared control cookies. Ferulic acid in wheat and barley cookie extracts, and quercetin and kaempferol-3-glucoside in wheat cookies with 15% blackcurrant were the dominant phenolic acids. Cellular antioxidant activity was higher in samples with blackcurrant inclusion when evaluated in a cancer cell HepG2 model. Inhibition of cell proliferation was lower for the phenolic samples from cookies with blackcurrant addition. These samples suppressed the regulation of inflammatory cytokine IL-1β (about 3 to 4-fold), IL-6 (about 2-fold) and transcription signalling factor NF-kB (about 2-fold) and showed an up-regulation of the satiety gene NUCB-2/nesfatin-1 (about 4-fold) in compared with control samples.