Aureobasidium pullulans volatile organic compounds as alternative postharvest method to control brown rot of stone fruits. (May 2020)

• Record Type: Journal Article
• Authors: Di Francesco, Alessandra; Di Foggia, Michele; Baraldi, Elena
• Is Part Of: Food microbiology. Volume 87(2020)
• Publication Date: 2020-05
• Subjects: Stone fruits -- Monilinia spp. -- Metabolites -- Aureobasidium pullulans -- ATR spectrometry; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2019.103395
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Volatile compounds produced by L1 and L8 strains were assayed against mycelia and conidia growth of Monilinia laxa, M. fructicola, M. polystroma, and M. fructigena of stone fruits. Results showed that volatile metabolites inhibited significantly pathogens growth, in particular M. fructigena mycelium growth (70% by L1 and 50% by L8) and M. fructicola conidia germination (85% by L1 and 70% by L8) compared to the control. Moreover, the antagonistic activity was enhanced by the addition of asparagine (120 mg L −1 ) in the culture media composition. Synthetic pure compounds were tested in vitro on pathogens mycelial and conidia growth and their EC50 values were estimated, confirming 2-phenethyl as the most active compound. For this reason 2-phenethyl and VOCs of both yeast strains were assayed in vivo on cherry, peach, and apricot fruits. Regarding peach fruit, both treatments, yeasts and pure compounds, displayed the best inhibiting action against all the pathogens especially against M. laxa (100% by L1, 84% by L8 and 2-phenethyl). ATR/IR spectroscopy analysis showed how VOCs produced by both strains increase the fruit waxes complexity reducing the pathogens attack so playing an essential role in the antagonistic activity of both yeast strains and on fruit structural composition. Highlights: Volatile organic compounds inhibited Monilinia spp. sporulation on stone fruits. Asparagine increase L1 and L8 antagonistic activity against Monilinia spp. growth. Volatile organic compounds increased stone fruits waxes complexity. 2-phenethyl was confirmed as the most active compounds produced by L1 and L8 strains.