Ultrasensitive quantitation of imidacloprid in vegetables by colloidal gold and time-resolved fluorescent nanobead traced lateral flow immunoassays. (1st May 2020)

• Record Type: Journal Article
• Authors: Tan, Guiyu; Zhao, Yajie; Wang, Mian; Chen, Xiaojiao; Wang, Baomin; Li, Qing X.
• Is Part Of: Food chemistry. Volume 311(2020)
• Publication Date: 2020-05-01
• Subjects: Lateral flow immunoassay -- Colloidal gold -- Time-resolved fluorescent -- Imidacloprid; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.126055
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Colloidal gold and time-resolved fluorescent nanobead for immunoassay. On-site quantitation of imidacloprid in vegetable by two lateral flow immunoassays. Comparison of two tracers for assay sensitivity at optimizing conditions. Lateral flow immunoassay is an alternative to enzyme-linked immunosorbent assay. Abstract: Imidacloprid is a highly effective insecticide, but its potential hazards to the environment and ecosystems have limited its use in many regions. A fast and sensitive analytical method would aid monitoring imidacloprid residues. The monoclonal antibody 4D9, colloidal gold and time-resolved fluorescent nanobeads (CGN and TRFN, respectively) were applied to develop a lateral flow immunoassay (LFIA) for imidacloprid detection in the present work. Both the optimized TRFN-LFIA and CGN-LFIA had a similar limit of detection of approximately 0.02 ng/mL. Use of the portable optical scanner with the TRFN-LFIA or CGN-LFIA could quantitate concentrations of imidacloprid in vegetable as sensitively as the enzyme-linked immunosorbent assay (ELISA). The LFIAs quantitative results of imidacloprid in commercial Chinese leeks were verified by the liquid chromatography-mass spectrometry with a R 2 of 0.91. The LFIAs are portable and simple and thus could fully replace the ELISA for onsite quantitation of imidacloprid residues in vegetables.