Multiple response optimization of a QuEChERS extraction and HPLC analysis of diclazuril, nicarbazin and lasalocid in chicken liver. (1st May 2020)

• Record Type: Journal Article
• Authors: Silva, Juan Manuel; Azcárate, Federico José; Knobel, Gaston; Sosa, Jesica Soledad; Carrizo, Daiana Belén; Boschetti, Carlos Eugenio
• Is Part Of: Food chemistry. Volume 311(2020)
• Publication Date: 2020-05-01
• Subjects: Coccidiostat residues -- Food safety -- Chicken liver -- QuEChERS -- Optimization -- Response surface methodology; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.126014
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Simultaneous determination of three coccidiostats in chicken liver by HPLC-UV/FL. Multi-residue extraction technique QuEChERS was used. Extraction procedure was optimized by experimental design. Analytical performance in accordance with food safety regulations. Abstract: A method for the simultaneous determination of three commonly used coccidiostats in chicken liver was developed, comprising a multi-residue QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction step, and a liquid chromatography-ultra violet-fluorescence (HPLC-UV/FL) analysis. The QuEChERS extraction was optimized using an experimental design approach that includes a screening step to obtain the critical variables, an optimization step using multiple response surface analysis and the calculation of a desirability parameter. The optimized method was validated with fortified samples, reaching an average recovery of 91% and an overall precision of 5.5% (mean of three analytes at three levels). Limits of detection calculated on fortified samples were 20 µg kg −1 for lasalocid, 15 µg kg −1 for nicarbazin and 120 µg kg −1 for diclazuril. These values resulted at least one order of magnitude lower than the maximum allowed residue limit (MRL) of the studied coccidiostats for chicken liver.