Identification of loci and molecular markers associated with Super Soft kernel texture in wheat. (May 2019)

• Record Type: Journal Article
• Authors: Kumar, Neeraj; Kiszonas, Alecia M.; Ibba, Maria Itria; Morris, Craig F.
• Is Part Of: Journal of cereal science. Volume 87(2019)
• Page Start: 286
• Page End: 291
• Publication Date: 2019-05
• Subjects: SKCS -- KASP -- SSR -- Marker-trait association -- Super soft -- Wheat; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2019.04.014
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: The preferential application of wheat flour in various end-use products is primarily driven by kernel texture (softness/hardness). Herein, a population of 268 F6 recombinant inbred lines (RILs) was developed by crossing the wheat cultivar Alpowa ('normal' soft) (SKCS HI = 20.2) with a related 'Super Soft' line (BC2SS163) (HI = −1.2). A set of 19 polymorphic markers including 16 Kompetitive allele specific PCR (KASP) and three simple sequence repeat (SSR) markers were targeted to three genomic regions (4BS, 1BS and 5AL) and used to genotype the RILs and parents. Four QTLs were detected for kernel texture using composite interval mapping (CIM) including one major QTL on 4BS, and three minor QTLs [5AL (2) and 1BS (1)]. Eight significant markers were identified using single marker-trait association (SMA) analysis and explained 3.8–28.0% phenotypic variation (PV). Two markers from the combined analyses were selected with the highest PV (8.0% and 36.7%). These two marker loci were independent and additive. RILs possessing both favorable (soft) alleles had an average SKCS hardness of 4.9 vs. 15.7 for the alternate haplotype. We conclude that the Super Soft trait is polygenic and that we have identified one of two or more major loci conferring this phenotype. Highlights: Marker-trait association is prerequisite for marker-assisted selection. CIM and SMA were conducted for a novel 'Super Soft' kernel texture trait using a soft white spring wheat F6 RIL population. Molecular markers (SSR and KASP) associated with kernel texture were identified. One major QTLs on 4BS, and three minor QTL on 1BS and 5AL affecting Super Soft kernel texture were identified.