Viability of Lactobacillus plantarum encapsulated with poly-γ-glutamic acid produced by Bacillus sp. SJ-10 during freeze-drying and in an in vitro gastrointestinal model. (September 2019)

• Record Type: Journal Article
• Authors: Jang, Won Je; Choi, Sun-Young; Lee, Jong Min; Lee, Ga Hye; Hasan, Md Tawheed; Kong, In-Soo
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 112(2019)
• Publication Date: 2019-09
• Subjects: Lactic acid bacteria -- Probiotic -- Encapsulation -- Poly-γ-glutamic acid -- Viability; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.05.120
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: In the present study, the probiotic Lactobacillus plantarum was encapsulated with 0.1%, 0.25%, or 0.5% 400-kDa poly-γ-glutamic acid (γ-PGA400 ) produced by Bacillus sp. SJ-10. The viability of the encapsulated cells was assessed under various stress conditions that are common to the processing and ingestion of probiotics, such as freeze-drying, exposure to simulated gastric juice (SGJ), and exposure to bile salt. During freeze-drying to make powder, L. plantarum levels decreased by 1.50 log colony forming units (CFU)/ml without encapsulation. When encapsulated with 0.5% γ-PGA400 under the same conditions, L. plantarum levels decreased by 0.19 log CFU/ml. In the SGJ condition (pH 2), all L. plantarum bacteria died within 1 h without encapsulation but exhibited the highest viability (decrease of 0.30 log CFU/ml) when encapsulated with 0.5% γ-PGA400 . All groups had a high survival rate in the bile salt condition (pH 5.9). In the intestinal adhesion test with Caco-2 cells, the highest rate of adherence was 35.9% when the cells were encapsulated with 0.25% γ-PGA400 . The present findings suggest that γ-PGA400 as an encapsulating material increases the viability of L. plantarum under various stress conditions. Highlights: γ-PGA-encapsulated L. planatarum demonstrated higher viability at the freeze-drying. Encapsulation increased L. planatarum survival against gastric juice condition. Encapsulated L. planatarum exhibited an enhanced adherence to Caco-2 cells. γ-PGA can increase the viability of LAB under various stress conditions.