An immobilized perylene diimide derivative for fucoidan purification from a crude brown algae extract. (February 2018)

• Record Type: Journal Article
• Authors: Zayed, Ahmed; Dienemann, Claudia; Giese, Christina; Krämer, Roland; Ulber, Roland
• Is Part Of: Process biochemistry. Volume 65(2018)
• Page Start: 233
• Page End: 238
• Publication Date: 2018-02
• Subjects: Fucoidan -- Perylene diimide derivative -- Beads -- Purification; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2017.10.012
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: An immoblized perylene diimide derivative is applied for fucoidan purification. Results showed more than 80% of available fucoidan was captured. Purified product demonstrated 97% purity and similar FTIR spectrum to reference fucoidan. Process automation was performed by a FPLC protocol. Purified fucoidan succeeded to prolong APTT and TT and showed potentuial anticoagulant activity. Abstract: A polycationic perylene diimide derivative was successfully immobilized to capture polysulphated fucoidan from a crude extract of F. vesiculosus . At a slightly acidic pH, immobilized perylene diimide derivative is positively charged and can form electrostatically-driven aggregates with more than 80% of fucoidan in only 16 h, compared to 44 h in recently developed immobilized toluidine blue. Purified fucoidan was identified by FTIR, which demonstrated typical IR bands in comparison with the commercial product. Moreover, it showed improved purity than crude type by a factor of 1.4 after purification. Pharmacologically, it prolonged APTT and TT, revealing a potential heparin-like anticoagulant activity by interfering with intrinsic and common blood coagulation pathway. Automated downstream and scale-up processes were further performed with developing a FPLC protocol. The new technique proved to be a competitor to immobilized toluidine blue for production of a high-grade fucoidan.