Black chokeberry (Aronia melanocarpa) polyphenols reveal different antioxidant, antimicrobial and neutrophil-modulating activities. (30th June 2019)

• Record Type: Journal Article
• Authors: Denev, Petko; Číž, Milan; Kratchanova, Maria; Blazheva, Denica
• Is Part Of: Food chemistry. Volume 284(2019)
• Page Start: 108
• Page End: 117
• Publication Date: 2019-06-30
• Subjects: Black chokeberry (Aronia melanocarpa) fruits -- Polyphenols -- Antioxidant activity -- Oxidative burst -- Neutrophils -- Antimicrobial properties; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.01.108
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Quercetin and epicatechin are the strongest antioxidants in black chokeberry. Proanthocyanidins contribute mostly to black chokeberry antioxidant activity. Black chokeberry polyphenols have mild effect on PMA-activated CL of neutrophils. Black chokeberry polyphenols have profound effect on OZP-activated CL of neutrophils. Black chokeberry proanthocyanidins are the strongest antimicrobial agents in the fruit. Abstract: The current study reports data on antioxidant, antimicrobial and neutrophil-modulating activities of different polyphenolic preparations from black chokeberry fruits: crude extract, purified extract standardized to 20% and 40% anthocyanins, and proanthocyanidins; as well as pure compounds (chlorogenic acid, cyanidin-3- O -galactoside, epicatechin, rutin and quercetin) present in black chokeberries. Minor phenolic components - quercetin and epicatechin showed the highest ORAC and TRAP antioxidant activity. Given the amount of individual phenolics in the fruits, proanthocyanidins are the major contributor to antioxidant activity of fresh black chokeberries. Studied polyphenols and preparations had no effect on the spontaneous chemiluminescence (CL) of human neutrophils and only mild effect on PMA-activated CL. Greater effects were observed on OZP-activated CL, being statistically significant (p < 0.05) for quercetin and rutin. The antimicrobial activity test against 10 pathogens showed that black chokeberry proanthocyanidins are the most potent antimicrobial agents in the fruit.