111 Fermentation of Soybean Meal Using a Novel Bacillus Subtilis Isolate to Improve Nutritive Value in Growing Pigs. (10th April 2018)

• Record Type: Journal Article
• Authors: Lu, Y; Akhtar, N; Zhu, C; Kiarie, E; Li, J
• Is Part Of: Journal of animal science. Volume 96(2018)Supplement 2
• Page Start: 58
• Page End: 59
• Publication Date: 2018-04-10
• Subjects: Bacillus subtilis -- Digestibility -- Fermentation; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/sky073.109
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: Soybean meal (SBM) fed to pigs contains a variety of antinutritional factors that impair the digestion of protein and utilization of nutrients. In the current study, a commercial SBM was subjected to solid state fermentation (SBM: water, 1:1; inoculum, 1%; temperature, 22-25°C) using a novel Bacillus subtilis CP-9 expressing high level of cellulase, xylanase and protease activities. Protein profile of the SBM after 48 h fermentation showed degradation of high molecular weight proteins including antigenic proteins into small-size peptides on sodium dodecyl sulfate polyacrylamide gel electrophoresis. To examine if fermentation altered the nutritive value of SBM, eight barrows (40 ± 2 kg BW) fitted with terminal ileal T-cannula were used. Two semi-purified corn starch–based diets were formulated with unfermented (UF) and fermented (F) SBM as the sole source of AA (min 18% CP, as-fed basis). Pigs were allocated in a two-period cross over design (n = 8) and were fed at 2.8 × maintenance energy requirement. Each period was 9 d; 5 d for adaptation, d 6 and 7 for grab fecal collection and d 8 and 9 for 8 h continuous ileal digesta collection. The diet was considered fixed effect whereas pig and period were considered random effects in statistical analysis. Pigs fed F-SBM had higher ( P <0.05) apparent ileal digestibility (AID) of CP (82.7 vs. 79.6%) and ash (46.3 vs. 43.0%) compared with pigs fed UF-SBM. There was no treatment effects ( P > 0.10) on AID of NDF and ATTD of acid detergent fiber (ADF), neutral detergent fiber (NDF) and gross energy (GE). In conclusion, fermentation of SBM by a novel Bacillus subtilis CP-9 increased ileal utilization of crude protein and minerals suggesting improved nutritive value in pigs.