Production and purification of antibody by immunizing rabbit with rice tungro bacilliform and rice tungro spherical viruses. (May 2018)

• Record Type: Journal Article
• Authors: Uda, M.N.A.; Gopinath, Subash C.B.; Hasfalina, C.M.; Faridah, S.; Bunawan, S.N.; Sabrina, W. Nur; Parmin, N.A.; Hashim, Uda; Afnan Uda, M.N.; Mazidah, M.
• Is Part Of: Process biochemistry. Volume 68(2018)
• Page Start: 37
• Page End: 42
• Publication Date: 2018-05
• Subjects: Tungro disease -- Rice tungro bacilliform virus -- Rice tungro spherical virus -- Antibody titer -- Enzyme-linked immunosorbent assay; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2018.02.019
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Antibody production against rice tungro bacilliform virus (RTBV) & rice tungro spherical virus (RTSV). Both pure viruses were used to immunize White New Zealand rabbits. Amount of the pure antibody in the titers was quantified. Anti-RTBV was higher at second bleed and anti-RTSV was at first bleed. Abstract: Rice tungro disease is the major disease caused by infection with the rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). In this study, New Zealand White rabbits were immunized with pure viruses for the production of antibodies against both species. The production of polyclonal antibodies against Tungro viral disease using ammonium sulfate precipitation and a protein A affinity column and their assessment are described. Two peaks were found from the protein A affinity column. Peak 1 represents the unbound compounds from the extracted serum and peak 2 represents antibody that bound to protein A, which was eluted using elution buffer. Peak 2 was collected for antibody titration. The amount of pure antibody in the titers was quantified by enzyme-linked immunosorbent assay (ELISA) to capture the tungro viruses. Antibody titer was analyzed by the ELISA method. For anti-RTBV, 1.696 mg/mL was highest at the second bleed and anti-RTSV was 2.3225 mg/mL was highest at the first bleed. These antibodies detected the tungro viral disease well and proved to be a potential probe for the detection of rice tungro disease.