Facilitating circular permutation using Restriction Free (RF) cloning. Issue 3 (29th November 2017)
- Record Type:
- Journal Article
- Title:
- Facilitating circular permutation using Restriction Free (RF) cloning. Issue 3 (29th November 2017)
- Main Title:
- Facilitating circular permutation using Restriction Free (RF) cloning
- Authors:
- Bandyopadhyay, Boudhayan
Peleg, Yoav - Abstract:
- Abstract: Circular permutation is a powerful tool to test the role of topology in protein folding and function. Previous methods for generating circular permutants were based on rearranging gene elements using restriction enzymes-based cloning. Here, we present a Restriction Free (RF) approach to achieve circular permutation which is faster and more cost-effective.
- Is Part Of:
- Protein engineering, design & selection. Volume 31:Issue 3(2018)
- Journal:
- Protein engineering, design & selection
- Issue:
- Volume 31:Issue 3(2018)
- Issue Display:
- Volume 31, Issue 3 (2018)
- Year:
- 2018
- Volume:
- 31
- Issue:
- 3
- Issue Sort Value:
- 2018-0031-0003-0000
- Page Start:
- 65
- Page End:
- 68
- Publication Date:
- 2017-11-29
- Subjects:
- circular permutation -- Restriction Free (RF) cloning
Protein engineering -- Periodicals
660.63 - Journal URLs:
- http://firstsearch.oclc.org ↗
http://peds.oxfordjournals.org/content/by/year ↗
http://ukcatalogue.oup.com/ ↗ - DOI:
- 10.1093/protein/gzx061 ↗
- Languages:
- English
- ISSNs:
- 1741-0126
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6936.055000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 12128.xml