In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes). Issue 11 (23rd October 2019)

• Record Type: Journal Article
• Authors: Morales, Diego; Tejedor-Calvo, Eva; Jurado-Chivato, Noelia; Polo, Gonzalo; Tabernero, María; Ruiz-Rodríguez, Alejandro; Largo, Carlota; Soler-Rivas, Cristina
• Is Part Of: Food & function. Volume 10:Issue 11(2019)
• Page Start: 7325
• Page End: 7332
• Publication Date: 2019-10-23
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/c9fo01744e
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Herein, two shiitake fractions were obtained: the ergosterol-enriched fraction exerted higher in vitro hypocholesterolemic activity and the β-glucan-enriched fraction reduced the cholesterol levels in mice. Abstract : Herein, a supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes under the following conditions: a temperature of 40 °C, pressure of 225 bar, reaction time of 1–5 h, and the flow rate of 20 L h −1 for recirculated CO2 . Moreover, ergosterol (ERG) and the SFE extract (SFE) with highest ergosterol concentration were microemulsified and submitted to in vitro digestion to study their ability to displace cholesterol from dietary mixed micelles (DMMs). ERG was also mixed with a β-glucan-enriched (33.5%) extract (BGE) obtained from L. edodes to investigate the synergies between them; the results indicated that all these extracts (including BGE without ERG) could reduce the cholesterol levels in the DMMs. However, when ERG and SFE were simultaneously administered to mice with a hypercholesterolemic diet, no significant differences in the serum cholesterol levels were detected as compared to the case of the control. However, when only BGE was administrated to another mice model previously induced with hypercholesterolemia, significant reduction in the cholesterol levels was noticed.