4‐Methylcatechol, a Flavonoid Metabolite with Potent Antiplatelet Effects. Issue 20 (7th August 2019)

• Record Type: Journal Article
• Authors: Applová, Lenka; Karlíčková, Jana; Warncke, Paul; Macáková, Kateřina; Hrubša, Marcel; Macháček, Miloslav; Tvrdý, Václav; Fischer, Dagmar; Mladěnka, Přemysl
• Is Part Of: Molecular nutrition & food research. Volume 63:Issue 20(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-08-07
• Subjects: 4‐methylcatechol -- aggregation -- flavonoids -- metabolites -- platelets -- pyrogallol; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.201900261
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Intake of flavonoids from the diet can be substantial, and epidemiological studies suggest that these compounds can decrease the incidence of cardiovascular diseases by involvement with increased platelet aggregation. Although parent flavonoids possess antiplatelet effects, the clinical importance is disputable due to their very low bioavailability. Most of them are metabolized by human colon bacteria to smaller phenolic compounds, which reach higher plasma concentrations than the parent flavonoids. In this study, a series of 29 known flavonoid metabolites is tested for antiplatelet potential. Methods and results: Four compounds appear to have a biologically relevant antiplatelet effect using whole human blood. 4‐Methylcatechol (4‐MC) is clearly the most efficient being about 10× times more active than clinically used acetylsalicylic acid. This ex vivo effect is also confirmed using a potentially novel in‐vivo‐like ex ovo hen's egg model of thrombosis, where 4‐MC significantly increases the survival of the eggs. The mechanism of action is studied and it seems that it is mainly based on the influence on intracellular calcium signaling. Conclusion: This study shows that some flavonoid metabolites formed by human microflora have a strong antiplatelet effect. This information can help to explain the antiplatelet potential of orally given flavonoids. Abstract : Flavonoids are metabolized by microflora into small phenolics. These small phenolics are absorbed. They include 4‐methylcatechol and pyrogallol. Both of them inhibit platelet aggregation ex vivo and they also improve survival in an in‐vivo‐like model of thrombosis.