Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29. (November 2019)

• Record Type: Journal Article
• Authors: Schmid, Maximilian T.; Song, Hyunjeong; Raschbauer, Michaela; Emerstorfer, Florian; Omann, Markus; Stelzer, Franz; Neureiter, Markus
• Is Part Of: Process biochemistry. Volume 86(2019)
• Page Start: 9
• Page End: 15
• Publication Date: 2019-11
• Subjects: Bacillus megaterium -- Desugarized sugar beet molasses -- Poly(3-hydroxybutyrate) -- Halophilic fermentation -- Sugar industry by-product; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.08.001
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Cost effective production of poly(3-hydroxybutyrate) (P(3HB)). Bacillus megaterium uyuni S29 produces 60 g P(3HB) per 100 g cell dry mass. Desugarized beet molasses is an inexpensive substrate for P(3HB) production. Simple and robust process for (P(3HB)) production. P(3HB) polymer consisting of two fractions with a molecular mass of 722 and 189 kDa. Abstract: Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L −1 with a P(3HB) content of 0.6 g g −1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn ) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L −1 h −1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.