Effects of fermented milk treatment on microbial population and metabolomic outcomes in a three-stage semi-continuous culture system. (15th October 2018)

• Record Type: Journal Article
• Authors: Cha, Kwang Hyun; Lee, Eun Ha; Yoon, Hyo Shin; Lee, Jae Ho; Kim, Joo Yun; Kang, Kyungsu; Park, Jin-Soo; Jin, Jong Beom; Ko, GwangPyo; Pan, Cheol-Ho
• Is Part Of: Food chemistry. Volume 263(2018)
• Page Start: 216
• Page End: 224
• Publication Date: 2018-10-15
• Subjects: Fermented milk -- Gut microbiota -- In vitro colon simulation -- MiSeq -- NMR-based metabolomics; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.04.095
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A fermented milk product was treated using an in vitro colonic fermentation. Three colon compartments showed an apparent microbial and metabolomic distinction. Fermented milk treatment induced an increase in butyrate-producing bacteria. There were clear changes in colonic primary metabolome after fermented milk treatment. Abstract: We investigated the impact of a fermented milk product on gut microbiota and their metabolism in 3 different conditions of the colon with a systemic viewpoint. An in vitro semi-continuous anaerobic cultivation was used to assess the colon compartment-specific influence of fermented milk, followed by a multiomics approach combining 16S rDNA amplicon sequencing and nuclear magnetic resonance (NMR) spectroscopy. The microbiome profiling and metabolomic features were significantly different across three colon compartments and after fermented milk treatment. Integrative correlation analysis indicated that the alteration of butyrate-producing microbiota ( Veillonella, Roseburia, Lachnospira, and Coprococcus ) and some primary metabolites (butyrate, ethanol, lactate, and isobutyrate) in the treatment group had a strong association with the fermented milk microorganisms. Our findings suggested that fermented milk treatment significantly affected microbial population in an in vitro cultivation system as well as the colonic metabolome in different ways in each of colon compartment.