Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates. (1st September 2018)

• Record Type: Journal Article
• Authors: Mudgil, Priti; Kamal, Hina; Yuen, Gan Chee; Maqsood, Sajid
• Is Part Of: Food chemistry. Volume 259(2018)
• Page Start: 46
• Page End: 54
• Publication Date: 2018-09-01
• Subjects: Dipeptidyl peptidase IV inhibition -- Pancreatic α-amylase inhibition -- Pancreatic lipase inhibition -- Camel milk proteins -- Bioactive peptides -- Alcalase -- Papain -- Bromelain; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.03.082
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Camel milk protein hydrolysates (CMPHs) effectively inhibited DPP-IV and lipase. 20 and 3 novel peptides were identified from A9 and B9 derived CMPHs, respectively. 7 and 2 peptides from A9 and B9 qualified to be PPL inhibitors, respectively. KDLWDDFKGL in A9 and MPSKPPLL in B9 were the potent α-amylase inhibitory peptides. Abstract: In-vitro inhibitory properties of peptides released from camel milk proteins against dipeptidyl peptidase-IV (DPP-IV), porcine pancreatic α-amylase (PPA), and porcine pancreatic lipase (PPL) were studied. Results revealed that upon hydrolysis by different enzymes, camel milk proteins displayed dramatic increase in inhibition of DPP-IV and PPL, but slight improvement in PPA inhibition was noticed. Peptide sequencing revealed a total of 20 and 3 peptides for A9 and B9 hydrolysates respectively, obtained the score of 0.8 or more on peptide ranker and were categorized as potential DPP-IV inhibitory peptides. KDLWDDFKGL in A9 and MPSKPPLL in B9 were identified as most potent PPA inhibitory peptide. For PPL inhibition only 7 and 2 peptides qualified as PPL inhibitory peptides from hydrolysates A9 and B9, respectively. The present study report for the first time PPA and PPL inhibitory and only second for DPP-IV inhibitory potential of protein hydrolysates from camel milk.