Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Koksal, Zeynep; Kalin, Ramazan; Gulcin, Ilhami; Ozdemir, Hasan
• Is Part Of: International journal of food properties. Volume 21:Issue 1(2018)
• Page Start: 385
• Page End: 394
• Publication Date: 2018-01-01
• Subjects: Plant peroxidase -- Radish (Raphanus sativus) -- Turnip (Brassica rapa) -- Pesticides -- Enzyme inhibition; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664.0705
• DOI: 10.1080/10942912.2018.1424197
• Languages: English
• ISSNs: 1094-2912

Abstract

ABSTRACT: The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2, 4-d -acid dimethylamine, fenoxaprop- p -ethyl, glyphosate isopropylamine, haloxyfop- p -methyl, cypermethrin, λ-cyhalothrin, and dichlorvos on the peroxidase purified from turnip ( Brassica rapa L.) and black radish ( Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, λ-cyhalothrin showed the strongest inhibitory effect against turnip peroxidase ( K i : 1.23 × 10 −2 ± 0.21 × 10 −2 mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase ( K i : 2.14 × 10 −2 ± 0.08 × 10 −2 mM) as competitive inhibition.