Bioactivities of peptide fractions derived from proteolytic enzyme-injected Hanwoo longissimus muscle in a model system. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Seol, Kuk-Hwan; Choe, Juhui; Kim, Hyun-Jin; Hwang, Jin-Taek; Lee, Mooha; Jo, Cheorun
• Is Part Of: International journal of food properties. Volume 21:Issue 1(2018)
• Page Start: 205
• Page End: 212
• Publication Date: 2018-01-01
• Subjects: Bioactivity -- Crude peptide fraction -- Protease type XIII -- Thermolysin -- Hanwoo beef; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664.0705
• DOI: 10.1080/10942912.2018.1440241
• Languages: English
• ISSNs: 1094-2912

Abstract

ABSTRACT: In this study, crude peptide fractions from Hanwoo loins were released by injecting with proteolytic enzymes [no enzymes (control); protease type XIII (E1); thermolysin (E2); and combination of E1 and E2 (E3)] and their bioactivities were determined. The peptides derived from E2-injected Hanwoo loin exhibited the highest angiotensin I–converting enzyme (ACE) inhibitory activity and vitamin C equivalents antioxidant capacity among the treatments. The released peptide by treatment of E2 and E3 had similar ( P > 0.05) inhibitory activity in HT29 cancer cell viability compared with luteolin as a positive control and non-cytotoxic effect on normal cell (3T3-L1). Therefore, the released peptide fraction from thermolysin (E2)-injected Hanwoo beef might contain potent bioactive peptides with ACE inhibitory and antioxidative activity and inhibition effect on certain cancer cell viability.