Potential biotechnological properties of an exopolysaccharide produced by newly isolated Bacillus tequilensis-GM from spontaneously fermented goat milk. (May 2019)

• Record Type: Journal Article
• Authors: Abid, Yousra; Azabou, Samia; Joulak, Ichrak; Casillo, Angela; Lanzetta, Rosa; Corsaro, Maria Michela; Gharsallaoui, Adem; Attia, Hamadi
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 105(2019)
• Page Start: 135
• Page End: 141
• Publication Date: 2019-05
• Subjects: Bacillus tequilensis-GM -- Exopolysaccharide -- Thermostability -- Emulsifying properties -- Metal adsorption; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2019.02.005
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: In the present work, an exopolysaccharide (EPS) was produced by a newly isolated Bacillus tequilensis -GM ropy strain. Results showed that EPS-GM was produced simultaneously with the growth phase and reached its maximum (2.9 g/L) when sucrose was used as carbon source. According to GC-MS and HPLC-SEC results, the recovered EPS-GM was composed by fructose, glucose, mannose, galactose, galactosamine and N -acetyl-glucosamine, with high molecular weight ranging between 1.8 and 2.5 × 10 6 Da. Different properties of EPS-GM were tested. Results showed that crude EPS-GM has several potential features, including high thermostability evaluated by DSC (with a melting point of 222 °C), good emulsifying properties and stability at both neutral and acidic pH, and high capacities to adsorb heavy metal ions (94.96% of Fe 2+, 97.66% of Zn 2+ and 98.24% of Cu 2+ ) making it promising to be used in different industrial fields. Highlights: EPS-GM was produced simultaneously with the growth phase of B. tequilensis -GM. EPS-GM exhibited high thermostability. EPS-GM showed good emulsifying properties at both neutral and acidic pH. EPS-GM was able to adsorb high levels of heavy metal ions.