Isoflavone accumulation and the metabolic gene expression in response to persistent UV-B irradiation in soybean sprouts. (15th January 2020)

• Record Type: Journal Article
• Authors: Lim, You Jin; Jeong, Ho Young; Gil, Chan Saem; Kwon, Soon-Jae; Na, Jong Kuk; Lee, Chanhui; Eom, Seok Hyun
• Is Part Of: Food chemistry. Volume 303(2020)
• Publication Date: 2020-01-15
• Subjects: UV-B ultraviolet B -- NO nitric oxide -- GmMYB Glycine max myeloblastosis viral oncogene homolog -- CHS chalcone synthase -- CHI chalcone isomerase -- IFS isoflavone synthase -- OD optical density -- HPLC reverse-phased high performance liquid chromatography -- DAF-2 DA 4, 5-diaminofluorescein diacetate -- PAL phenylalanine ammonia lyase -- CHS, chalcone synthase IFL, isoflavone lyase -- HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid -- NADPH nicotinamide adenine dinucleotide phosphate hydrogen -- GmCHR Glycine max chalcone reductase -- qRT-PCR quantitative real-time polymerase chain reaction -- GmUGT Glycine max UDP-glycosyltransferase -- GmMT Glycine max malonyltransferase; Isoflavone -- Metabolic gene expression -- Nitric oxide -- Soybean sprouts; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125376
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Six of 12 isoflavones in soybean sprouts were increased by UV-B irradiation. Malonyl genistin did not increase in dark but did in the sprouts grown under UV-B. UV-B specific chalcone synthetic genes were GmCHS 6, 7, and 8. Most isoflavone metabolic genes were highly activated in response to UV-B 24–36 h. Abstract: This study investigated the effects of persistent ultraviolet B (UV-B) irradiation on isoflavone accumulation in soybean sprouts. Three malonyl isoflavones were increased by UV-B. Malonylgenistin specifically accumulated upon UV-B exposure, whereas the other isoflavones were significantly increased under both dark conditions and UV-B exposure. The results of isoflavone accumulation to UV-B irradiation time were observed as following: acetyl glycitin rapidly increased and then gradually decreased; malonyl daidzin and malonyl genistin were highly accumulated within an intermediate period; genistein and daidzin were gradually maximized; daidzin, glycitin, genistein, and malonyl glycitin did not increase; and glycitin, acetyl daidzin, and acetyl genistin exhibited trace amounts. Transcriptional analysis of isoflavonoid biosynthetic genes demonstrated that most metabolic genes were highly activated in response to UV-B 24 and UV-B 36 treatments. In particular, it was found that GmCHS6, GmCHS7, and GmCHS8 genes among the eight known genes encoding chalcone synthase were specifically related to UV-B response.