Black rice anthocyanins embedded in self-assembled chitosan/chondroitin sulfate nanoparticles enhance apoptosis in HCT-116 cells. (15th December 2019)

• Record Type: Journal Article
• Authors: Liang, Tisong; Zhang, Zhentao; Jing, Pu
• Is Part Of: Food chemistry. Volume 301(2019)
• Publication Date: 2019-12-15
• Subjects: CH chitosan -- CS chondroitin sulfate -- ACN anthocyanin -- TGA thermogravimetric analysis -- FTIR Fourier transform infrared spectrophotometry; Self-assembly -- Nanoparticles -- Anthocyanin -- Chitosan -- Apoptosis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125280
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Bioploymer nanoparticles loaded anthocyanins under mild conditions and short time. The formation of nanoparticles based on the electrostatic interaction of CH and CS. The nanoparticles present reasonable particle size, and having high thermal stability. The nanoparticles showed higher biological activity than anthocyanins alone. The effects of nanoparticles on cells were evaluated via cell viability and apoptosis. Abstract: Self-assembled nanoparticles using the biopolymers chitosan (CH) and chondroitin sulfate (CS) were developed to improve the biological activity of anthocyanin (ACN). The 86.32 ± 0.15% (w/w) of ACN was incorporated into ACN/CH/CS nanoparticles, with the particle size of 350.1 ± 0.99 nm in diameter (i.d.) and 42.55 ± 0.54 in zeta potential (mV). Morphological study and thermogravimetric analysis suggested that the ACN/CH/CS nanoparticles exhibited heterogeneous morphology and high thermal stability. Significant increases in apoptosis by 12.1% and 35.1% were observed with 0.05 mg/ml ACN and ACN/CH/CS nanoparticles in the HCT-116 cell line, indicating that the nanoparticle system led to significant increase in apoptosis ( p < 0.05). Structural changes in mitochondria caused by ACN/CH/CS nanoparticles indicated that the nanoparticles had negative impacts on mitochondria. These results showed that nanoparticles could potentially be used as a carrier system to improve the efficacy of ACN.