Effects of inhibiting starch branching enzymes on molecular and crystalline structures of starches from endosperm different regions in rice. (15th December 2019)

• Record Type: Journal Article
• Authors: Lin, Lingshang; Huang, Jun; Zhang, Long; Zhang, Changquan; Liu, Qiaoquan; Wei, Cunxu
• Is Part Of: Food chemistry. Volume 301(2019)
• Publication Date: 2019-12-15
• Subjects: Rice -- Starch branching enzyme -- Starch -- Endosperm regional tissue -- Molecular structure -- Crystalline structure; AAC apparent amylose content -- AC amylose content -- DAF days after flowering -- GLXN rice cultivar Guang-ling-xiang-nuo -- GPC gel-permeation chromatography -- HPAEC high-performance anion-exchange chromatography -- SBE starch branching enzyme -- TQ rice cultivar Te-qing -- WX9915 rice cultivar Wu-xiang 9915 -- XRD X-ray diffraction; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125271
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Three rice cultivars and their transgenic lines with inhibition of SBEs were studied. Different regions of endosperm had similar starch structure in wild-type rice. Amylose increased from the interior to exterior of endosperm with inhibition of SBEs. Inhibiting SBEs gradually changed amylopectin from the inner to outer of endosperm. Different regions of endosperm with inhibition of SBEs had different crystallinities. Abstract: Mature endosperm was separated regionally into different parts in three rice cultivars, Te-qing (TQ), Wu-xiang 9915 (WX9915) and Guang-ling-xiang-nuo (GLXN), and their transgenic lines with inhibition of starch branching enzyme I and IIb (SBEI/IIb − ). Within the three wild-type cultivars, starches from endosperm different regions showed similar molecular and crystalline structures. However, in rices with inhibition of SBEs, amylopectin short branch-chain content and branching degree gradually decreased, but amylopectin B3+ chain content and average chain length increased gradually from the interior to exterior of endosperm. The amylose content gradually increased from the interior to exterior of endosperm in TQ- and WX9915-SBEI/II − lines. From the interior to exterior of endosperm, starch changed gradually from CC - to CB -type in TQ-SBEI/II − line and from CA - to CC -type in GLXN-SBEI/II – line, and remained CA -type in WX9915-SBEI/II – line. These results provided some information for quality breeding and utilizations of rice with inhibition of SBE.