Determination of acetamiprid, imidacloprid, and spirotetramat and their relevant metabolites in pistachio using modified QuEChERS combined with liquid chromatography-tandem mass spectrometry. (1st February 2018)

• Record Type: Journal Article
• Authors: Faraji, Mohammad; Noorbakhsh, Roya; Shafieyan, Hooshang; Ramezani, Mohammadkazem
• Is Part Of: Food chemistry. Volume 240(2018)
• Page Start: 634
• Page End: 641
• Publication Date: 2018-02-01
• Subjects: Acetamiprid -- Imidacloprid -- Spirotetramat -- High-performance liquid chromatography-tandem mass spectrometry -- QuEChERS -- Pistachio; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.08.012
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: QuEChERS extraction procedure for pesticides determination in pistachio samples. Acetamiprid, imidacloprid, and spirotetramat and their relevant metabolites were determined. Efficient extraction and clean-up with negligible matrix effect were obtained. Satisfactory linearity, recoveries, accuracy and precision were obtained. Abstract: A QuEChERS based methodology was developed for the simultaneous identification and quantification of acetamiprid, imidacloprid, and spirotetramat and their relevant metabolites in pistachio by liquid chromatography-tandem mass spectrometry for the first time. First, sample extraction was done with MeCN:citrate buffer:NaHCO3 followed by phase separation with the addition of MgSO4 :NaCl. The supernatant was then cleaned by a primary-secondary amine (PSA), GCB, and MgSO4 . The proposed method provides a linearity in the range of 5–200 µg L −1, and the linear regression coefficients were higher than 0.99. LOD and LOQ were obtained to be 2 and 5 µg kg −1 for the studied insecticides, respectively, with the exception of imidacloprid-olefin (5 and 10 µg kg −1 ). Acceptable recoveries (91–110%) were obtained for all the analytes with good intra- and inter-precisions (0.4 ≥ RSD ≤11.0). The method was then used for the pistachio samples collected from a field trial to estimate the maximum residue limits (MRLs) in next step.