Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues. (1st January 2020)

• Record Type: Journal Article
• Authors: Peng, Dapeng; Kavanagh, Owen; Gao, Haijiao; Zhang, Xiya; Deng, Sijun; Chen, Dongmei; Liu, Zhenli; Xie, Changqing; Situ, Chen; Yuan, Zonghui
• Is Part Of: Food chemistry. Volume 302(2019)
• Publication Date: 2020-01-01
• Subjects: Surface plasmon resonance biosensor -- 3-Methyl-quinoxaline-2-carboxylic acid -- Olaquindox -- Residues -- Swine tissues; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.04.022
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A new SPR method for the determination of MQCA had been reported. The developed SPR method is the first report for MQCA in animal tissues. The SPR method can serve as an effective screening tool in any routine laboratory. Abstract: To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg −1 in swine muscle and 2.7 µg kg −1 in swine liver, which are lower than the EU recommended concentration (10 µg kg −1 ). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.