Nutraceutical potential of hemp (Cannabis sativa L.) seeds and sprouts. (1st October 2018)

• Record Type: Journal Article
• Authors: Frassinetti, Stefania; Moccia, Eleonora; Caltavuturo, Leonardo; Gabriele, Morena; Longo, Vincenzo; Bellani, Lorenza; Giorgi, Gianluca; Giorgetti, Lucia
• Is Part Of: Food chemistry. Volume 262(2018)
• Page Start: 56
• Page End: 66
• Publication Date: 2018-10-01
• Subjects: oxygen radical absorbance capacity ORAC -- 2, 2-diphenyl-1 picrylhydrazyl radical scavenger assay DPPH -- cellular antioxidant activity in red blood cells CAA-RBC; DPPH, free radical (PubChem CID: 2735032) -- DCFH-DA 2′, 7′-dichlorofluorescein diacetate (PubChem CID: 104913) -- Glutamic acid (PubChem CID: 33032) -- Caffeoyltyramine (PubChem CID: 9994897) -- Cannabisin A (PubChem CID: 15086398) -- Cannabisin B (PubChem CID: 101631692); Cannabis sativa L. -- Seeds -- Germination -- Antioxidant capacity -- Anti-mutagenic activity -- Metabolomic fingerprint -- Functional food; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.04.078
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Cannabis sativa L. seeds and sprouts showed a high in vitro and ex vivo antioxidant activity. Cannabis sativa L. seeds and sprouts exerted antimutagenic activity in yeast Saccharomyces cerevisiae. Phytochemical compounds were characterized and identified via high resolution mass spectrometry in negative ion mode. Seeds germination increased the total content of bioactive compounds. Hemp seeds and sprouts may represent a potential functional food. Abstract: In this study the antioxidant effect of Cannabis sativa L. seeds and sprouts (3 and 5 days of germination) was evaluated. Total polyphenols, flavonoids and flavonols content, when expressed on dry weight basis, were highest in sprouts; ORAC and DPPH ( in vitro assays), CAA-RBC (cellular antioxidant activity in red blood cells) and hemolysis test ( ex vivo assays) evidenced a good antioxidant activity higher in sprouts than in seeds. Untargeted analysis by high resolution mass spectrometry in negative ion mode allowed the identification of main polyphenols (caffeoyltyramine, cannabisin A, B, C) in seeds and of ω-6 (linoleic acid) in sprouts. Antimutagenic effect of seeds and sprouts extracts evidenced a significant decrease of mutagenesis induced by hydrogen peroxide in Saccharomyces cerevisiae D7 strain. In conclusion our results show that C. sativa seeds and sprouts exert beneficial effects on yeast and human cells and should be further investigated as a potential functional food.