Effect of enzymatic hydrolysis on bioactive properties and allergenicity of cricket (Gryllodes sigillatus) protein. (1st October 2018)

• Record Type: Journal Article
• Authors: Hall, Felicia; Johnson, Philip E.; Liceaga, Andrea
• Is Part Of: Food chemistry. Volume 262(2018)
• Page Start: 39
• Page End: 47
• Publication Date: 2018-10-01
• Subjects: Cricket protein hydrolysates -- Bioactive properties -- Antioxidant -- ACE inhibition -- DPP-IV inhibition -- Allergenicity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.04.058
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Whole crickets were hydrolyzed with alcalase then gastrointestinal proteases. Hydrolysates displayed good ACE-, DPP-IV inhibition, and antioxidant activity. Bioactivity increased, in most cases, after simulated gastrointestinal digestion. IgE reactivity to tropomyosin decreased in hydrolysates with 60–85% degree of hydrolysis. Abstract: Food-derived bioactive peptides have gained attention for their role in preventing chronic diseases. Edible insects are viable sources of bioactive peptides owing to their high protein content and sustainable production. In this study, whole crickets ( Gryllodes sigillatus) were alcalase-hydrolyzed to a degree of hydrolysis (DH) ranging from 15 to 85%. Antioxidant activity, angiotensin converting enzyme (ACE), and dipeptidyl peptidase-4 (DPP-IV)- inhibition of the cricket protein hydrolysates (CPH) were evaluated before and after simulated gastrointestinal digestion (SGD). Antioxidant activity was similar among CPH, whereas ACE and DPP-IV inhibition was greater (p < 0.05) in CPH with 60–85% DH. Bioactivity improved after SGD. CPH allergenicity was evaluated using human shrimp-allergic sera. All sera positively reacted to tropomyosin in the unhydrolyzed cricket and CPH with 15–50% DH, whereas 60–85% DH showed no reactivity. In conclusion, CPH (60–85% DH) had the greatest bioactive potential and lowest reactivity to tropomyosin, compared with other CPH and the unhydrolyzed control.