Development of a screening analytical method for the determination of non-dioxin-like polychlorinated biphenyls in chicken eggs by gaschromatography and electron capture detection. Issue 9 (2nd September 2019)

• Record Type: Journal Article
• Authors: Nardelli, Valeria; D'Amico, Valeria; Casamassima, Francesco; Gesualdo, Giuseppe; Li, Donghao; Marchesiello, Wadir M.V.; Nardiello, Donatella; Quinto, Maurizio
• Is Part Of: Food additives & contaminants. Volume 36:Issue 9(2019)
• Page Start: 1393
• Page End: 1403
• Publication Date: 2019-09-02
• Subjects: NDL-PCBs -- chicken eggs -- accelerated solvent extraction (ASE) -- gas chromatography/electron capture detection -- solid phase extraction (SPE) -- validation; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2019.1627002
• Languages: English
• ISSNs: 1944-0049

Abstract

ABSTRACT: A sensitive and reproducible screening analytical method is here proposed for the determination of six non dioxin-like polychlorinated biphenyls (NDL-PCBs, congener 28, 52, 101, 138, 153, 180) in chicken eggs based on accelerated solvent extraction (ASE) procedure for the fat extraction and determination, a solid phase extraction (SPE) sample clean-up process, and a gas chromatography – electron capture detection (GC-ECD) analysis. The optimized chromatographic separation, in less than 25 min, returned good responses for the six NDL-PCBs in the range of 2.5–60.0 µg L −1, with correlation coefficients always higher than 0.9995. Instrumental limits of detection were between 0.08–0.35 µg L −1, corresponding to 0.05 and 0.23 ng g −1 fat in the matrix, while method detection limits, calculated on spiked egg samples, ranged from 1.6 to 3.5 ng g −1 fat. The method has been extensively validated in terms of selectivity, sensitivity, recovery, precision, ruggedness and measurement uncertainty, following the European Directives. Graphical Abstract: