Enhanced nutraceutical potential of gamma irradiated black soybean extracts. (15th April 2018)

• Record Type: Journal Article
• Authors: Krishnan, Veda; Gothwal, Santosh; Dahuja, Anil; Vinutha, T.; Singh, Bhupinder; Jolly, Monica; Praveen, Shelly; Sachdev, Archana
• Is Part Of: Food chemistry. Volume 245(2018)
• Page Start: 246
• Page End: 253
• Publication Date: 2018-04-15
• Subjects: CVD chronic vascular diseases -- ROS reactive oxygen species -- kGy kilogrey -- ACN acetonitrile -- FA formic acid -- TPC total phenolic content -- TFA total isoflavone content -- TAC total antioxidant capacity -- C3G cyanidin-3-glucoside -- FRAP ferric ion reducing power assay -- DPPH 2, 2-diphenyl-1-picrylhydrazyl radical assay -- RSC radical-scavenging capacity -- DCFH-DA 2′, 7′ dichloro fluorescein diacetate -- TPTZ 2, 4, 6-tripyridyl-S-triazine -- F3H flavanone 3-hydroxylase -- DFR dihydroflavanol 4-reductase -- ANR anthocyanidin reductase -- UF3GT UDP-g1ucose:flavonoid 5-O-glucosyltransferase -- EPR electron paramagnetic resonance; Soybean -- Gamma irradiation -- Cyanidin-3-glucoside -- Cytoprotective effect; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2017.10.099
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Nutraceutical enhancement potential of gamma irradiation is proposed. Low dose of gamma irradiation enhanced anthocyanins, especially cyanidin-3-glucoside (C3G). In vitro assay confirmed the cyto-protective role of irradiated black soybean extracts. Abstract: Radiation processing of soybean, varying in seed coat colour, was carried out at dose levels of 0.25, 0.5 and 1 kGy to evaluate their potential anti-proliferative and cytoprotective effects in an in vitro cell culture system. Irradiated and control black (Kalitur) and yellow (DS9712) soybean extracts were characterized in terms of total phenolics, flavonoids and anthocyanins, especially cyanidin-3-glucoside (C3G). Using an epithelial cell line, BEAS-2B the potential cytoprotective effects of soybean extracts were evaluated in terms of intracellular ROS levels and cell viability. The most relevant scavenging effect was found in Kalitur, with 78% decrease in ROS, which well correlated with a 33% increase in C3G after a 1 kGy dose. Results evidenced a correspondence between in vitro antioxidant activity and a potential health property of black soybean extracts, exemplifying the nutraceutical role of C3G. To our knowledge this study is the first report validating the cytoprotective effects of irradiated black soybean extracts.