Effect of refrigerated storage on betacyanin composition, antibacterial activity of red pitahaya (Hylocereus polyrhizus) and cytotoxicity evaluation of betacyanin rich extract on normal human cell lines. (May 2018)

• Record Type: Journal Article
• Authors: Yong, Yi Yi; Dykes, Gary; Lee, Sui Mae; Choo, Wee Sim
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 91(2018)
• Page Start: 491
• Page End: 497
• Publication Date: 2018-05
• Subjects: Antimicrobial -- Betalains -- Cold storage -- Post-harvest storage -- Red dragon fruit; Betanin -- Hylocerenin -- Isobetanin -- Phyllocactin; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2018.01.078
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Red pitahaya fruit is rich in purplish-red color water-soluble betacyanins. The effect of refrigerated storage at 4 °C on the betacyanin composition of red pitahaya was monitored each day, for one week, using HPLC. The betacyanin content of red pitahaya had increased by 57.2% after storage for six days at 4 °C. From day 2 to day 7, the phyllocactin content decreased from 45.4 ± 1.5% to 38.3 ± 3.1%, whereas the betanin content increased from 37.9 ± 1.6% to 42.7 ± 1.5%. Antimicrobial activity was determined using the broth microdilution assay and expressed as minimum inhibitory concentration (MIC). As compared to the freshly harvested fruits (MIC values: 50, 000–>50, 000 μg mL −1 ), fruits that were stored for six days at 4 °C demonstrated better antimicrobial activity (MIC values: 3130–6250 μg mL −1 ) against 10 pathogenic Gram-positive and 6 Gram-negative bacteria. Betacyanin rich extract was also examined against normal human cell lines (HEK-293 and THP-1) and found to be not cytotoxic at the levels of 0.39–3.13 mg mL −1 . In summary, refrigerated storage for 6 days increased the betacyanin content, changed the betacyanin composition in red pitahaya and contributed to higher antimicrobial activity. Highlights: The betacyanin rich extract was considered not cytotoxic to normal human cells. The betacyanin content of red pitahaya increased after refrigeration for 6 days. The antibacterial activity of red pitahaya increased after refrigeration for 6 days.