Development and validation of an ultra-high performance supercritical fluid chromatography-photodiode array detection-mass spectrometry method for the simultaneous determination of 12 compounds in Piper longum L. (15th November 2019)

• Record Type: Journal Article
• Authors: Xu, Rongrong; Chen, Xiaoqing; Wang, Xing; Yu, Lan; Zhao, Wenwen; Ba, Yinying; Wu, Xia
• Is Part Of: Food chemistry. Volume 298(2019)
• Publication Date: 2019-11-15
• Subjects: Piper longum L. -- UHPSFC-MS -- Piperine -- Analogues -- Simultaneous determination -- Quality control; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.125067
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: 12 compounds were isolated, identified from P. longum L. An UHPSFC method was developed to analyze the mixture from P. longum in 8 min. Temperature, back-pressure and gradient were optimized simultaneously by DoE. 12 compounds from P. longum were determined simultaneously by UHPSFC-MS. 28 batches of P. longum from different producing areas were analyzed by PCA. Abstract: An ultra-high performance supercritical fluid chromatography-photodiode array detection-mass spectrometry (UHPSFC-MS) method for quality control of Piper longum L. has been developed and optimized. Hexane/isopropanol (70/30, v/v) was determined as the final injection solvent and methanol as the organic modifier. A design-of-experimental (DoE) approach was used to optimize column temperature, back-pressure and the gradient slope simultaneously using Trefoil CEL1 column. The back-pressure, temperature, flow rate were set at 130 bar, 32.5 °C and 1.0 mL/min, respectively. Positive electrospray ionization was used in the single ion monitoring mode. The 12 analytes were analyzed within 8 min using the optimized conditions. The linearities of the standard calibrations were satisfactory with coefficients of determination ( R 2 ) > 0.995. The recovery measured varied from 96.34% to 105.00% with relative standard deviation (RSD) ≤ 4.68%. The method was sensitive, reliable and effective, and successfully applied to simultaneous determination of 12 compounds in 28 batches of P. longum.