Effect of the Aqueous Extract of Lantana grisebachii Stuck Against Bioaccumulated Arsenic-Induced Oxidative and Lipid Dysfunction in Rat Splenocytes. (4th July 2019)

• Record Type: Journal Article
• Authors: Ramos Elizagaray, Sabina I.; Quiroga, Patricia L.; Pérez, Roberto D.; Sosa, Carlos; Pérez, Carlos A.; Bongiovanni, Guillermina A.; Soria, Elio A.
• Is Part Of: Journal of dietary supplements. Volume 16:Number 4(2019)
• Page Start: 401
• Page End: 407
• Publication Date: 2019-07-04
• Subjects: arsenicosis -- fatty acid -- lantana -- peroxidation -- spleen -- X-ray fluorescence; Dietary supplements -- Periodicals; Dietary Supplements -- Periodicals; 613.205
• DOI: 10.1080/19390211.2018.1470124
• Languages: English
• ISSNs: 1939-0211

Abstract

ABSTRACT: Arsenic (As) is a worldwide immunotoxic agent that is in contaminated waters and consumed by mammals. Phytotherapy may counteract its harmful effects. Lantana grisebachii Stuck (LG, Verbenaceae ) and its extract are proposed as protective, given vvits in vitro bioactivity. The aim was to determine the protective capacity of the aqueous LG extract on splenocytes exposed in vivo to arsenic. Splenocytes were obtained from an arsenicosis model (Wistar rats consuming orally 0 [control; C] or 5 mg/Kg/d of As) that received 0–100 mg/Kg/d of LG extract for 30 days. As content (total reflection X-ray fluorescence), fatty acid profile (gas chromatography), γ-glutamyl transpeptidase activity (Szasz method), peroxides (xylenol orange-based assay), and nitrites (Griess reaction) were then assayed in viable splenocytes. Data were analyzed with ANOVA and the Tukey's test ( p < .05). It was observed that the splenocytes contained 2.2 mg/Kg of this elemental arsenic. With γ-glutamyl transpeptidase inhibition and consequent triggering of hydroperoxides ( p < .05), it was observed to increase saturated fatty acids and alter lipid profiles. LG treatment avoided damaging effects with values similar to unexposed C ( p < .05), and cellular arsenic concentration ( p < .0001). In conclusion, the aqueous extract of L. grisebachii counteracted arsenic toxicity in rat splenocytes by preventing its cellular accumulation and induction of lipid and redox disturbances, which may impair immune function.