A facile water-induced complexation of lycopene and pectin from pink guava byproduct: Extraction, characterization and kinetic studies. (30th October 2019)

• Record Type: Journal Article
• Authors: Nagarajan, Jayesree; Krishnamurthy, Nagendra Prasad; Nagasundara Ramanan, Ramakrishnan; Raghunandan, Mavinakere Eshwaraiah; Galanakis, Charis M.; Ooi, Chien Wei
• Is Part Of: Food chemistry. Volume 296(2019)
• Page Start: 47
• Page End: 55
• Publication Date: 2019-10-30
• Subjects: Pink guava decanter -- Water -- Lycopene -- Pectin -- Colloidal complex; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2019.05.135
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A simple, fast and cost-effective method for recovery of lycopene and pectin. Complexation of lycopene-pectin from pink guava decanter was induced by water. Extracted lycopene attained 99% of purity level and was in all-trans configuration. Antioxidant activity of the extracted lycopene was preserved. Time-course experimental data was best fitted with two-site kinetic model. Abstract: The redfleshed pulp discarded from pink guava puree industry is a rich source of lycopene and pectin. In this study, we developed a facile extraction process employing water as the primary extraction medium to isolate the lycopene and pectin from pink guava decanter. When the decanter was suspended in water, the complexation of lycopene and pectin formed the cloudy solution, where the colloidal complexes were recovered through centrifugation. The presence of lycopene and pectin in the complex was confirmed by the spectroscopic, microscopic and chromatographic analyses. The lycopene fractionated from the complexes had a purity level of 99% and was in all- trans configuration. The colloidal complexes yielding the highest concentration of lycopene was obtained at pH 7, 1% (w/v) solid loading and 25 °C. The experimental data of time-course extraction of lycopene-pectin complex were best fitted with two-site kinetic model, hinting the fast- and slow-release phases in the extraction process.