Soybean peroxidase-catalysed removal of benzidines from water. Issue 4 (22nd February 2016)

• Record Type: Journal Article
• Authors: Altahir, Bahaa Malik; Feng, Wei; Jasim, Hadi Hassan; Taylor, Keith E.; Biswas, Niharendu; Bewtra, Jatinder K.; Jassim, Sabah A. A.
• Is Part Of: Journal of environmental engineering and science. Volume 10:Issue 4(2015)
• Page Start: 73
• Page End: 80
• Publication Date: 2016-02-22
• Subjects: environmental engineering -- industrial wastes -- waste management & disposal; Environmental engineering -- Periodicals; Sanitary engineering -- Periodicals; Environmental engineering -- Canada -- Periodicals; Environnement, Technique de l' -- Périodiques; Technique sanitaire -- Périodiques; Environnement, Technique de l' -- Canada -- Périodiques; Environmental engineering; Sanitary engineering; Canada; Electronic journals; Computer network resources; Periodicals; 628.05
• DOI: 10.1680/jenes.15.00018
• Languages: English
• ISSNs: 1496-2551

Abstract

Abstract : Crude soybean peroxidase (SBP), isolated from soybean seed coats (hulls) at unusually low concentrations, catalyses the oxidative polymerisation of hazardous aqueous benzidine and its 3, 3′-dichloro, 3, 3′-dimethyl and 3, 3′-dimethoxy derivatives in the presence of hydrogen peroxide. The optimum operating conditions for oxidation of 0·10 mM benzidine were investigated. At pH 5, the hydrogen peroxide-to-substrate concentration ratio was 1·5 and the minimum SBP concentration required to achieve at least 95% conversion of the benzidine in synthetic wastewater was 0·43 mU/ml. Progress curves were established for the conversion of the four substrates, and apparent first-order rate constants were derived. Enzyme-catalysed polymerisation with SBP and subsequent removal of the polymeric products generated can provide an alternative means to conventional methods for treating many aromatic wastewater pollutants, including the benzidines studied here.