Polysaccharide isolated from Chinese jujube fruit (Zizyphus jujuba cv. Junzao) exerts anti-inflammatory effects through MAPK signaling. (January 2018)

• Record Type: Journal Article
• Authors: Zhan, Rui; Xia, Long; Shao, Jinhui; Wang, Cheng; Chen, Daofeng
• Is Part Of: Journal of functional foods. Volume 40(2018)
• Page Start: 461
• Page End: 470
• Publication Date: 2018-01
• Subjects: CCK8 Cell Counting Kit-8 -- COX-2 cyclooxygenase-2 -- ERK1/2 extracellular signal-regulated kinase 1/2 -- IFN-γ interferon gamma -- iNOS inducible nitric oxide synthase -- IL-17 interleukin-17 -- JNK c-Jun N-terminal kinase -- LPS lipopolysaccharide -- MAPKs mitogen-activated protein kinases -- NO nitric oxide -- PGE2 prostaglandin E2 -- PMP 1-phenyl-3-methyl-5-pyrazolone -- TLR4 Toll-like receptor 4 -- TNF-α tumor necrosis factor-α -- NSAIDs non-steroidal anti-inflammatory drugs; Lipopolysaccharide (PubChem CID: 11970143) -- Acetone (PubChem CID: 180) -- Phenol (PubChem CID: 996) -- sulfuric acid (PubChem CID: 1118); Zizyphus jujuba cv. Junzao -- Anti-inflammatory activity -- Pectic polysaccharide -- Cytokines -- Lipopolysaccharide; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.11.026
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: A pectic polysaccharide was isolated from Zizyphus jujuba cv. Junzao. The structure of ZJP was characterized by chemical analysis, FT-IR, NMR and GPC-MALLS. ZJP showed strong anti-inflammatory activity on RAW 264.7 cells. ZJP could be used to treat inflammatory disease or in health products. Abstract: A peptic polysaccharide (ZJP) was isolated from Zizyphus jujuba cv. Junzao by hot water extraction and chromatographic purification. Structural characterization showes that ZJP has a weight-average molecular weight of 153.3 kDa and consists of galacturonic acid. The linkage type of ZJP was proven to be 1, 4-linked D-Gal p A by methylation, NMR and MS analysis. The anti-inflammatory effects of ZJP were tested on RAW264.7 macrophages. ZJP was found to significantly suppress nitric oxide (NO) production and decrease levels of pro-inflammatory cytokines such as COX-2, TNF-α, IFN-γ and IL-17. Molecular profiling showed that ZJP could inhibit phosphorylation of the signaling proteins p-38 and JNK, but not ERK, revealing ZJP exert anti-inflammatory effects through inhibition of NF-κB and p38/JNK MAPK signaling. This study suggests that ZJP can be used to treat inflammatory diseases or as a supplement to health products.