Imaging an Adapted Dentoalveolar Complex. (6th September 2011)

• Record Type: Journal Article
• Authors: Herber, Ralf-Peter; Fong, Justine; Lucas, Seth A.; Ho, Sunita P.
• Other Names: Gülekon Nadir Academic Editor.
• Is Part Of: Anatomy research international. Volume 2012(2012)
• Publication Date: 2011-09-06
• Subjects: Anatomy -- Periodicals; 611.005
• DOI: 10.1155/2012/782571
• Languages: English
• ISSNs: 2090-2743

Abstract

Abstract : Adaptation of a rat dentoalveolar complex was illustrated using various imaging modalities. Micro-X-ray computed tomography for 3D modeling, combined with complementary techniques, including image processing, scanning electron microscopy, fluorochrome labeling, conventional histology (H&E, TRAP), and immunohistochemistry (RANKL, OPN) elucidated the dynamic nature of bone, the periodontal ligament-space, and cementum in the rat periodontium. Tomography and electron microscopy illustrated structural adaptation of calcified tissues at a higher resolution. Ongoing biomineralization was analyzed using fluorochrome labeling, and by evaluating attenuation profiles using virtual sections from 3D tomographies. Osteoclastic distribution as a function of anatomical location was illustrated by combining histology, immunohistochemistry, and tomography. While tomography and SEM provided past resorption-related events, future adaptive changes were deduced by identifying matrix biomolecules using immunohistochemistry. Thus, a dynamic picture of the dentoalveolar complex in rats was illustrated.