Zinc chelated morin promotes osteoblast differentiation over its uncomplexed counterpart. (July 2019)

• Record Type: Journal Article
• Authors: Vimalraj, Selvaraj; Rajalakshmi, Subramaniyam; Saravanan, Sekaran; Thirumalai, Deepak; Kadarkarai, Murugan; Rajkumar, A.Vasanthi; Dhanasekaran, Anuradha
• Is Part Of: Process biochemistry. Volume 82(2019)
• Page Start: 167
• Page End: 172
• Publication Date: 2019-07
• Subjects: Morin-zinc complex -- Chick embryo model -- Non-toxic -- Osteoblasts -- Differentiation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.04.008
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Zinc complex of morin mono/bis complexes have been synthesized. Zinc complexes found to be non-toxic upto 60μM in in vitro and in vivo toxicity assay. Zinc complexes increased osteoblast differentiation. Zinc complex of morin bis complexes showed high potency in osteoblast differentiation. Abstract: Flavonoids are commonly occurring polyphenolic compounds with a wide array of bioactive properties, including its positive role on osteoblast differentiation and bone formation. Their profound ability to chelate various metal ions supported its candidature as a novel biomaterial for tissue engineering applications. We aimed to investigate the role of Morin-zinc complexes (Morin mono complex, [Zn(morin)(H2 O)] and Morin bis complex, [Zn(morin)2 ]) in promoting osteoblast differentiation at both molecular and cellular levels. In vitro biocompatibilities of the synthesized complexes (20–200 μM) were assessed with MG-63, H9c2 and C3H10T1/2 cells and chick embryo model was used to assess in vivo toxicity. Both Morin and Morin zinc complexes were found to be non-toxic at 60μM concentration. Furthermore, at cellular level, the complexes increased alkaline phosphatase activity (ALP) and calcium deposition. At molecular level it increased Runx2, type 1 collagen mRNAs expression and enhanced the secretion of osteocalcin (OC) and osteonectin (ON) proteins. Among the synthesized complexes, [Zn(morin)2 ] exhibited more pronounced effects compared to naïve Morin and [Zn(morin)] complex. Overall, the current study proposes that Morin and Morin zinc complexes could be used for bone tissue engineering applications.