Bacterial cellulose grows with a honeycomb geometry in a solid-state culture of Gluconacetobacter xylinus using polyurethane foam support. (July 2019)

• Record Type: Journal Article
• Authors: Quintana-Quirino, Mariana; Morales-Osorio, Cindy; Vigueras Ramírez, Gabriel; Vázquez-Torres, Humberto; Shirai, Keiko
• Is Part Of: Process biochemistry. Volume 82(2019)
• Page Start: 1
• Page End: 9
• Publication Date: 2019-07
• Subjects: Gluconacetobacter xylinus -- Bacterial cellulose -- Solid-state culture -- Polyurethane foam -- Biofilm; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.04.023
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Polyurethane foam allows bacterial cellulose production in solid state culture. The highest yield was 2.46 g L-1 with 0.125 cm3 foam and 15% inoculum for 96 h. Polyurethane foam presents high water storage capacity and oxygen diffusion. Bacteria synthesize the biopolymer as biofilm adhered to the solid matrix scaffold. Abstract: The polyurethane foam (PUF) allowed the growth of Gluconacetobacter xylinus for prompt production of bacterial cellulose (BC) due to high water storage capacity that let high water activity, as well as diffusion of oxygen through the PUF pores. PUF was studied in two sizes cut (0.13 cm 3 ) and minced (0.08 cm 3 ). Additionally, the study assessed the level of inoculum on the growth and the formation of BC. The highest yield of BC was 2.46 g L -1 of cellulose achieved with cut foam and 15% inoculum at 96 h. Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD) and solid state 13 C cross polarization and magic angle spinning nuclear magnetic resonance (CP/MAS NMR) analyses evidence crystalline structure type I for the produced microbial cellulose. According to scanning electron microscopy (SEM) analysis, the bacteria synthesized the biopolymer as a biofilm adhered to the solid support; thereby BC displays a honeycomb geometry.