Extraction of keratin from waste chicken feathers using sodium sulfide and l-cysteine. (July 2019)

• Record Type: Journal Article
• Authors: Pourjavaheri, Firoozeh; Ostovar Pour, Saeideh; Jones, Oliver A.H.; Smooker, Peter M.; Brkljača, Robert; Sherkat, Frank; Blanch, Ewan W.; Gupta, Arun; Shanks, Robert A.
• Is Part Of: Process biochemistry. Volume 82(2019)
• Page Start: 205
• Page End: 214
• Publication Date: 2019-07
• Subjects: Feathers -- Green chemistry -- Protein -- Polymers; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2019.04.010
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Novel research of the chicken feather keratin applications into new products – namely a hair conditioner and leather treatment cream. Extraction of poultry chicken feather visa Na2 S and L-Cysteine. Characterisation of extracted keratin by Raman, FTIR, NMR spectroscopies, SDS-PAGE and thermogravimetry. Abstract: Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide andl -cysteine. The yield of extracted keratin from sodium sulfide andl -cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α -helix and ß -sheet based keratin could be extracted from CF using sodium sulfide andl -cysteine with high yields. This is the first report of CF keratin extraction usingl -cysteine.