Methodology to nanoencapsulate hepatoprotective components from Picrorhiza kurroa as food supplement. (1st March 2015)

• Record Type: Journal Article
• Authors: Jia, Dewei; Barwal, Indu; Thakur, Shloka; Yadav, Subhash C.
• Is Part Of: Food bioscience. Volume 9(2015:Mar.)
• Page Start: 28
• Page End: 35
• Publication Date: 2015-03-01
• Subjects: Poly(d, l-lactide) (PubChem CID: 612) -- Pluronic-F-68 (PubChem CID: 24751) -- Methanol (PubChem CID: 887) -- Acetone (PubChem CID: 180) -- Picroside I (PubChem CID: 6440892) -- Picroside II (PubChem CID: 9849283) -- Sodium dihydrogen phosphate (PubChem CID: 23672064) -- Di-sodium hydrogen phosphate (PubChem CID: 24203) -- Water (PubChem CID: 962) -- Phosphotungstic acid (PubChem CID: 16212977); Heptoprotective -- Picrorhiza kurroa -- Nanoencapsulation -- Polylactic acid -- Pluronic-F-68; HPLC high performance liquid chromatography -- DLS dynamic light scattering -- RP-HPLC reverse phase high performance liquid chromatography -- PLA polylactic acid -- FTIR Fourier transform infrared spectroscopy -- TEM transmission electron microscopy; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2014.10.005
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Picrorhiza kurroa root and rhizome powder of extract is well-recognized neutraceutical for healthy liver functioning. The major hepatoprotective components (picrosides I and II) of P. kurroa extract shows poor intestinal absorption and poor bioavailability due to sparingly aqueous solubility. To improve bioavailability of these biomolecules, a nanoformulation of this extract has been developed with pluronic-F-68 copolymer based biodegradable PLA nanoparticles by nanoprecipitation method. This method showed high encapsulation efficiency as 60.1±2.8% (for picroside I) and 67.2±7.4% (for picroside II). The hydrodynamic (by zeta size) and actual size (by TEM) revealed homogenously rounded nanoparticles of size 174±34 nm and 154±12 nm respectively. This formulation showed an initial burst and half release up to 27±3 h, followed by a sustained release (up to 210±10 h). Nanoformulation of this traditional herbal extract provides neutraceutical with value addition property for better hepato-protection by enhancing intestinal absorption and bioavailability.