Event-specific qualitative and quantitative detection of three genetically modified papaya events using a single standard reference molecule. (September 2015)

• Record Type: Journal Article
• Authors: Kim, Jae-Hwan; Park, Saet-Byul; Roh, Hyo-Jeong; Woo, Hong-Bae; Shin, Min-Ki; Moon, Gui Im; Hong, Jin-Hwan; Zhang, Dabing; Kim, Hae-Yeong
• Is Part Of: Food control. Volume 55(2015:Sep.)
• Page Start: 127
• Page End: 132
• Publication Date: 2015-09
• Subjects: Genetically modified papaya -- Standard reference plasmid -- Qualification -- Quantification -- Polymerase chain reaction; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2015.02.048
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: A novel standard reference plasmid (pGEM-PAPAYA3) was constructed as a positive control and reference standard for event-specific qualitative and quantitative detection of genetically modified (GM) papaya (55-1, 16-0-1, and Huanong No.1). The plasmid pGEM-PAPAYA3 contained the specific fragments of papaya endogenous reference gene chymopapain and the 3 GM papaya events. The qualitative PCR assay using pGEM-PAPAYA3 was established with a limit of detection correlating to approximately 5–50 copies of papaya haploid genomes. In the quantitative PCR assay, the square regression coefficients ( R 2 ) ranged from 0.993 to 0.997. The standard deviation and relative standard deviation values for repeatability ranged from 0.04 to 0.25 and 0.05%–0.86%, respectively. The method was used to test 11 papaya products purchased from Thailand, China, the Philippines and the USA, and the results revealed 2 varieties of GM papaya in 5 of the 11 samples tested. These results indicate the developed detection methods with the standard reference plasmid are applicable for identifying 3 GM papaya events. Highlights: Event-specific PCR detection methods were established for 3 GM papaya events. A standard reference plasmid was developed as a calibrator for the GM papaya detection. We showed GM papaya 55-1 and 16-0-1 in 5 of 11 papaya samples tested.