Effective clean-up and ultra high-performance liquid chromatography–tandem mass spectrometry for isoflavone determination in legumes. (1st May 2015)

• Record Type: Journal Article
• Authors: Vila-Donat, Pilar; Caprioli, Giovanni; Maggi, Filippo; Ricciutelli, Massimo; Torregiani, Elisabetta; Vittori, Sauro; Sagratini, Gianni
• Is Part Of: Food chemistry. Volume 174(2015)
• Page Start: 487
• Page End: 494
• Publication Date: 2015-05-01
• Subjects: UHPLC–MS/MS ultra high-performance liquid chromatography–tandem mass spectrometry -- SPE solid phase extraction -- ESI electrospray ionisation -- RP-HPLC-DAD reverse phase high-performance liquid chromatography diode array detector -- HPLC-UV high-performance liquid chromatography ultraviolet detector -- GC–MS gas chromatography–mass spectrometry; Legumes -- Phytoestrogens -- Isoflavones -- Clean-up -- SPE-UHPLC–MS/MS; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2014.11.047
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Legumes excellent source of nutrients and phytochemicals such as isoflavones. Analysis of isoflavones in legumes by freezing clean up step and UHPLC–MS/MS system. Correlation between isoflavone content in lentils and the altitude of the fields where they are grown. Abstract: Legumes are an excellent source of macronutrients and phytochemicals as isoflavones. The aim of this work was to develop a new analytical method for determining five isoflavone compounds, three of which are aglycons, namely daidzein, genistein, biochanin A, and two of which, daidzin and genistin, are glycosilated, in lentils and other pulses, using an effective clean-up system and UHPLC–MS/MS (triple quadrupole) method. The recoveries obtained by spiking the lentil samples with a standard mixture of isoflavones at three levels of fortification (5, 25 and 100 μg kg −1 ) were in the range of 54.4–111.1%, 68.6–91.1%, and 84.4–114%, respectively. The method was applied to analyse 48 lentil samples from central Italy and pulses for determining the isoflavone content, which was found to range from 1.1 to 95.6 μg kg −1 .